Sequencing Services

MR DNA specializes in any type of amplicon sequencing.

 

From 16s, 18s, ITS, functional genes to any type of custom primer amplicon assay.

We have an extensive inhouse assay (primer) collection for 16s sequencing, 18s sequencing,  ITS sequencing, functional genes such as nirS, nifH, dsr,  pufM, nosZ, HMC,  and many more.  if you have a custom assay we inexpensively set it up for you.

 

Amplicon Sequencing Prices.

 

MR DNA has all the major sequencing platforms and we have a large selection of amplicon sequencing programs ..  Everything can be customized to the needs of the customer, only limited by the capabilities of the sequencing technologies.

 

Any amplicons (bTEFAP® services) such as 16s, 18s, ITS, functional or custom assays.. if you have an amplicon with custom primers we can help sequence it.

 

Illumina miseq and hiseq amplicons

examples for any of our hundreds of inhouse assays

***2x300bp PE illumina 20,000 sequence diversity assays

$85/assay 1-50 assays (note: for projects < 10 assays per library, a $100 library fee is added),

$80 for 50-100 assays,

$75 for 100-150 assays,

$70 for 150-300,

$65 for > 300 assays.

additional discounts for very large projects also

 

PGM (ion torrent) amplicons

***300bp 515F bacterial + archaeal or ITS1-2 fungal diversity PGM assays with nominal 15-20,000 reads/assay for $60/assay (any size project)

 

PAC BIO SEQUEL:

NEW:  Long read full 16s ~1400bp amplicon diversity average 5k reads per sample (minimum 20 samples) for $220/sample

 

LONG read ITS1-4 sequencing for fungi

 

long read 18s  1700bp spanning many variable regions  (several options available)

 

long read custom assays from 600bp up to 8000bp  (custom assay setup costs

range from $15-$20/barcode)

 

 

Barcoded amplicon sequencing has become a popular sequencing solution for scientists interested in a specific genomic region.  Amplicon sequencing combined with next-generation sequencing allows for thousands of amplicons across many samples to be prepared simultaneously and indexed within hours and often within a single-run. Due to its popularization, barcoded amplicon sequencing can now be completed through a variety of different processes utilizing several different technologies. MR DNA has been at the forefront of this technology and through calculated fine tuning, we are proud to offer an inexpensive sequencing method known as bTEFAP®. Originally designed to employ 16s eubacterial primers to be sequenced via pyrosequencing technology, we have expanded its low cost sequencing capabilities to include Archaea, Fungi, and functional gene primers compatible to be sequenced across all of our sequencing platforms. The increased flexibility and versatility of bTEFAP® gives us the opportunity to provide our customers with the lowest sequencing cost possible.

In The News

A New Species

The one bacteria most everyone is familiar with, and maybe without even knowing they are...Clostridium difficile. This diarrhea causing bacteria, C. difficile, may be splitting into two. Researchers from the Wellcome Sanger Institute have just completed a large scale research project evaluating this pesky little bug with specific concern to its presence within hospital environments. This study involved sequencing the DNA of 906 strains of C. difficile collected across 33 countries, and as a result of this collective effort, scientists suggest that a new bacterial species is emerging, which is currently known as C. difficile clade A.

 

This potential new species was found in 70% of samples collected in hospital patients and of course one of the key differences in C. difficile clade A is its resistance to hospital disinfectants. It is clear that DNA sequencing is elucidating the seemingly infinite number of micro-environments that exist in our world today. It is no secret that the word "microbiome" is all the buzz in our circles recently and for good reason. As technology increases and cost decreases, we are discovering just how much our world is influenced by the bacteria that surrounds us everyday. Microbiology, Ecology, Immunology, Agricultural Science, Citizen Science etc. the list could go on and on with regards to the research areas that are growing as a result of DNA Sequencing. Speciation events such as the one discoverd in Clostridium difficile is just one example as to how the power of DNA sequencing is making large scal research studies possible.

 

Reference:

Sanger. Diarrhoea-causing bacteria adapted to spread in hospitals. Retrieved from https://www.sanger.ac.uk/news/view/diarrhoea-causing-bacteria-adapted-spread-hospitals