Amplicon Sequencing (bTEFAP®)

Sequencing Services

MR DNA specializes in any type of amplicon sequencing.

 

From 16s, 18s, ITS, functional genes to any type of custom primer amplicon assay.

We have an extensive in-house assay (primer) collection for 16s sequencing, 18s sequencing,  ITS sequencing, functional genes such as nirS, nifH, dsr,  pufM, nosZ, HMC,  and many more.  if you have a custom assay we inexpensively set it up for you.

 

Amplicon Sequencing Prices.

 

MR DNA has all the major sequencing platforms and we have a large selection of amplicon sequencing programs ..  Everything can be customized to the needs of the customer, only limited by the capabilities of the sequencing technologies.

 

Any amplicons (bTEFAP® services) such as 16s, 18s, ITS, functional or custom assays.. if you have an amplicon with custom primers we can help sequence it.

 

Illumina MiSeq and NovaSeq amplicons

examples for any of our hundreds of inhouse assays

***2x300bp PE illumina 20,000 sequence diversity assays

$80/assay 1-20 assays (note: for projects < 10 assays per library, a $100 library fee is added),

$75 for 20 - 50 assays,

$70 for 50 -100 assays,

$65 for 100-150,

$60 for > 150 assays.

additional discounts for very large projects also

 

Ion S5 XL (Ion Torrent) amplicons

All Ion S5 XL projects are being migrated to the Illumina MiSeq and Illumina NovaSeq Sequencing Platforms

All economy sequencing projects previously completed using the Ion S5 will now be completed using the Illumina MiSeq and NovaSeq at no additional cost.

  • The same 515F-806R and ITS1-2 primers will continue to be made available for use on all Illumina sequencing platforms
  • Contact us to discuss additional Illumina specific primer sets and pricing options

 

300bp 515F bacterial + archaeal or ITS1-2 fungal diversity PGM assays with nominal 15-20,000 reads/assay for $60/assay (any size project)

 

>400bp bacterial, archaeal, or ITS fungal diversity assays with nominal 15-20,000 reads/assay starting at $85/assay and continues to follow the Illumina MiSeq price scale above.

 

 

PAC BIO SEQUEL:

Sequel long read amplicon sequencing for 16s, 18s, ITS and custom amplicons

MR DNA now accepts any size project (projects with < 10 samples do have a $500 indexing fee and a $20/sample additional PCR replication fee.)

 

Low average coverage ( < 500 sequences average per sample)  for $50/sample

Medium coverage (average 5000 sequences per sample)  for $150/sample

High coverage  10K average sequences scale for $260/sample

Custom scales can have as much data per sample as desired in multiples of 10K with price linearly increased.

 

custom assays from 700bp - 3000bp or larger of course have a barcoding that is typically $15/barcode

 

DNA Extraction / RNA Extraction:

--DNA Extraction starting at $25 / sample (depending on service requested and sample type submitted)

--RNA Extraction starting between $30 - $70 / sample (depending  on service requested and sample type submitted)

 

1)    2x300bp Illumina:  (Price per sample can be lower, depending on how many samples, more samples = lower price per sample) .. you can of course request greater depth. (we can take smaller projects but it may be small delay as we gather enough samples to coordinate with small projects).   We can currently have a variety of 16s assays, fungi, archaea and a few functional genes.. Our system allows us to inexpensively setup new assays and custom assays also

 

NOTE: CUSTOM assay prices are generally only an additional $10-$20/barcode (one time setup fee).  If you need a custom assay we are happy to get them ordered and tested.

 

2) LONG READ AMPLICONS up to 8000bp,   with 5,000 avg sequence per sample.  One popular application of this is the near full length 16s sequencing using primers 27F-1492R. Projects less than 10 samples may include an additional library fee. Please contact us for more details.

 

 

 

 

Barcoded amplicon sequencing has become a popular sequencing solution for scientists interested in a specific genomic region.  Amplicon sequencing combined with next-generation sequencing allows for thousands of amplicons across many samples to be prepared simultaneously and indexed within hours and often within a single-run. Due to its popularization, barcoded amplicon sequencing can now be completed through a variety of different processes utilizing several different technologies. MR DNA has been at the forefront of this technology and through calculated fine tuning, we are proud to offer an inexpensive sequencing method known as bTEFAP®. Originally designed to employ 16s eubacterial primers to be sequenced via pyrosequencing technology, we have expanded its low cost sequencing capabilities to include Archaea, Fungi, and functional gene primers compatible to be sequenced across all of our sequencing platforms. The increased flexibility and versatility of bTEFAP® gives us the opportunity to provide our customers with the lowest sequencing cost possible.